About Bioziny

BioZiny is a water based Nano Engineered Antibacterial Coating for Your Home. It is nano zinc oxide (ZnO) based, easy to apply, transparent, aqueous Coating for high touch surfaces at home, hospitals, medical centers, schools, busses, trains, airplanes, busses and other public places like shopping malls, Supermarkets, children’s play areas etc.
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What is the active ingredient in BioZiny?

What is the active ingredient in BioZiny?

The active ingredient in BioZiny is surface modified Nano Zinc Oxide. Nano Zinc Oxide has demonstrated antibacterial properties against both Gram Positive and Gram Negative Bacteria.

The antibacterial and antifungal properties of nano-zinc oxide have been thoroughly studied and the results are ubiquitous in scientific literature. The list of publications listed below extensively talks about the Antibacterial and Antimicrobial Properties of Nano ZnO.

Zinc Ions (Zn2+) exhibit antimicrobial activity. In aqueous suspension or when in contact with water, Zinc Oxide particles release Zinc Ions (Zn2+) that contributes to elimination of bacteria.

Nano Zinc Oxide acts on bacteria on one or more of the following ways:


Very High Specific Surface Area:

Particles are less than 100nm in diameter thus having more pronounced antibacterial activities than large particles since the small size and high specific surface area allow better interaction with bacteria.


Release of Antimicrobial Ions:

Zinc Ions (Zn2+) exhibit antimicrobial activity. In aqueous suspension or when in contact with water, Zinc Oxide particles release Zinc Ions (Zn2+) that contributes to elimination of bacteria.


Direct Interaction of Nano ZnO particles with Bacteria:

The interaction between ZnO nanoparticles and bacterial cells is caused by electrostatic forces. The global charge of bacterial cells at biological pH values is negative, due to the excess of carboxylic groups, which are dissociated and provide a negative charge to the cell surface. On the other hand ZnO nanoparticles have a positive charge. As a result, opposite charges between the bacteria and Nanoparticles generate electrostatic forces, leading to a strong bind between the nanoparticles and the bacteria surface leading to disruption of the cell walls causing internalization of the nanoparticles in the bacterial cells. Nano ZnO causes great damage to the bacterial cells like disorganization of the cell walls, alteration of morphology and internal cell content leakage.



The surface of Nano ZnO has been considered to be abrasive due to presence of surface defects such as edges and corners. The bacterial cells are also damaged by the abrasive surfaces of ZnO Nanoparticles.


Generation of Reactive Oxygen Species (ROS):

Being a semiconductor with a band gap of approx. 3.3 eV, under the influence of radiation with photon energy more than its band gap, leads to the formation of an electron hole (h+). The electron hole (h+) reacts with H2O molecules (from the suspension of ZnO) separating them into •OH and H+. In addition, O2 molecules dissolved in the medium are transformed into superoxide anion radicals (O2 ̇−), which in turn react with H+ to generate (HO2 •). Subsequently, this species collides with electrons producing hydrogen peroxide anions (HO2−). Thus, the hydrogen peroxide anion reacts with hydrogen ions to produce H2O2 molecules, which is a very strong oxidizing agent. H2O2 molecules elevate the membrane lipid peroxidation that causes membrane leakage

of reducing sugars, DNA, Proteins and reduces cell viability.



Paula Judith Perez Espitia, Nilda de Fátima Ferreira Soares, Jane Sélia dos Reis Coimbra & Nélio José de Andrade, Renato Souza Cruz, Eber Antonio Alves Medeiros (2012): Zinc Oxide Nanoparticles: Synthesis, Antimicrobial Activity and Food Packaging Applications. Food Bioprocess Technology (2012) 5:1447–1464

Li, J. H., Hong, R. Y., Li, M. Y., Li, H. Z., Zheng, Y., & Ding, J. (2009). Effects of ZnO nanoparticles on the mechanical and antibacterial properties of polyurethane coatings. Progress in Organic Coatings, 64(4), 504–509

Padmavathy, N., & Vijayaraghavan, R. (2008). Enhanced bioactivity of ZnO nanoparticles—an antimicrobial study. Science and Technology of Advanced Materials, 9(3), 035004

Xie, Y., He, Y., Irwin, P. L., Jin, T., & Shi, X. (2011). Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni. Applied and Environmental Microbiology, 77(7), 2325–2331.

Zhang, L., Ding, Y., Povey, M., & York, D. (2008). ZnO nanofluids—a potential antibacterial agent. Progress in Natural Science, 18(8), 939–944.

Zhang, L., Jiang, Y., Ding, Y., Povey, M., & York, D. (2007). Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofluids). Journal of Nanoparticle Research, 9(3), 479–489.

Jones, N., Ray, B., Ranjit, K. T., & Manna, A. C. (2008). Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiology Letters, 279(1), 71–76.

Ohira, T., Yamamoto, O., Iida, Y., & Nakagawa, Z. (2008). Antibacterial activity of ZnO powder with crystallographic orientation. Journal of Materials Science. Materials in Medicine, 19(3), 1407–1412

Brayner, R., Ferrari-Iliou, R., Brivois, N., Djediat, S., Benedetti, M. F.,& Fiévet, F. (2006). Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. Nano Letters, 6(4), 866–870.

Adams, L. K., Lyon, D. Y., & Alvarez, P. J. J. (2006). Comparative eco-toxicity of nanoscale TiO2, SiO2, and ZnO water suspensions. Water Research, 40(19), 3527–3532.

Emamifar, A., Kadivar, M., Shahedi, M., & Soleimanian-Zad, S. (2010). Evaluation of nanocomposite packaging containing Ag and ZnO on shelf life of fresh orange juice. Innovative Food Science & Emerging Technologies, 11(4), 742–748.

Eskandari, M., Haghighi, N., Ahmadi, V., Haghighi, F., & Mohammadi, S. R. (2011). Growth and investigation of antifungal properties of ZnO nanorod arrays on the glass. Physica B: Condensed Matter, 406 (1), 112–114.

Gordon, T., Perlstein, B., Houbara, O., Felner, I., Banin, E., & Margel, S. (2011). Synthesis and characterization of zinc/iron oxide composite nanoparticles and their antibacterial properties. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 374 (1–3), 1–8

He, L., Liu, Y., Mustapha, A., & Lin, M. (2011). Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum. Microbiological Research, 166(3), 207–215.

Hirota, K., Sugimoto, M., Kato, M., Tsukagoshi, K., Tanigawa, T., & Sugimoto, H. (2010). Preparation of zinc oxide ceramics with a sustainable antibacterial activity under dark conditions. Ceramics International, 36(2), 497–506.

Jalal, R., Goharshadi, E. K., Abareshi, M., Moosavi, M., Yousefi, A., & Nancarrow, P. (2010). ZnO nanofluids: green synthesis, characterization, and antibacterial activity. Materials Chemistry and Physics, 121(1–2), 198–201.

Kasemets, K., Ivask, A., Dubourguier, H.-C., & Kahru, A. (2009). Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae. Toxicology In Vitro, 23(6), 1116–1122.

Bhadra, P., Mitra, M. K., Das, G. C., Dey, R., & Mukherjee, S. (2011). Interaction of chitosan capped ZnO nanorods with Escherichia coli. Materials Science and Engineering: C, 31(5), 929–937.

Reddy, K. M., Feris, K., Bell, J., Wingett, D. G., Hanley, C., & Punnoose, A. (2007). Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Applied Physics Letters, 90(21), 213902

Sawai, J. (2003). Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay. Journal of Microbiological Methods, 54(2), 177–182.

Sawai, J., Shoji, S., Igarashi, H., Hashimoto, A., Kokugan, T., Shimizu, M., & Kojima, H. (1998). Hydrogen peroxide as an antibacterial factor in zinc oxide powder slurry. Journal of Fermentation and Bioengineering, 86(5), 521–522.

Roselli, M., Finamore, A., Garaguso, I., Britti, M. S., & Mengheri, E.(2003). Zinc oxide protects cultured enterocytes from the damage induced by Escherichia coli. Journal of Nutrition, 133(12), 4077– 4082.

Shi, L., Zhou, J., & Gunasekaran, S. (2008). Low temperature fabrication of ZnO—whey protein isolate nanocomposite. Materials Letters, 62(28), 4383–4385
Vicentini, D. S., Smania, A., Jr., & Laranjeira, M. C. M. (2010). Chitosan/poly (vinyl alcohol) films containing ZnO nanoparticles and plasticizers. Materials Science and Engineering: C, 30(4), 503–508.

Mizieli ́ nska, M.; Łopusiewicz, Ł.; M ̨e ̇zy ́ nska, M.; Bartkowiak, A. The influence of accelerated UV-A and Q-SUN irradiation on the antimicrobial properties of coatings containing ZnO nanoparticles. Molecules 2017, 22, 1556.

Azizi, S.; Ahmad, M.B.; Hussein, M.Z.; Ibrahim, N.A. Synthesis, Antibacterial and Thermal Studies of Cellulose Nanocrystal Stabilized ZnO-Ag Heterostructure Nanoparticles. Molecules 2013, 18, 6269–6280

Mizieli ́ nska, M.; Lisiecki, S.; Jotko, M.; Chodzy ́ nska, I.; Bartkowiak, A. The antimicrobial properties of polylactide films covered with ZnO nanoparticles-containing layers. Przem. Chem. 2015, 94, 1000–1003.

Noshirvani, N.; Ghanbarzadeh, B.; Mokarram, R.R.; Hashemi, M. Novel active packaging based on carboxymethyl cellulose-chitosan-ZnO NPs nanocomposite for increasing the shelf life of bread. Food Packag. Shelf Life 2017, 11, 106–114.

Oprea, A.E.; Pandel, L.M.; Dumitrescu, A.M.; Andronescu, E.; Grumezescu, V.; Chifiriuc, M.C.; Mogoanta, L.; Bal ̧seanu, T.-A.; Mogo ̧sanu, G.D.; Socol, G.; et al. Bioactive ZnO Coatings Deposited by MAPLE—An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces. Molecules 2016, 21, 220.

Silvestre, C.; Duraccio, D.; Marra, A.; Strongone, V.; Cimmino, S. Development of antimicrobial composite films based on isotactic polypropylene and cZnO particles for active food packaging.